Agrisera/TOR | Target of rapamycin/AS12 2608/,Agrisera,,Agrisera,Agrisera AB,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Agrisera/TOR | Target of rapamycin/AS12 2608/

产品编号：AS122608

市场价：¥5560.00

场地：美国(厂家直采)

产品分类：抗体类>一抗>多克隆抗体>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$278.00

品牌： Agrisera

公司：Agrisera AB

公司分类：

商品介绍


productinformation
Background		TOR(Targetofrapamycin)isanessentialcellgrowthregulatorthatcontrolsdevelopmentfromearlyembryotoseedproductioninresponsetonutrientavailABIlityinenvoronmentalstressconditions.Itbelongstothefamilyofphosphatidylinositol3-kinaseandaretargetsoftheantiproliferativedrugrapamycin.AtTORisexpressedinembryos,endospermandmeristems.ActsthroughthephosphorylationofdownstreameffectorsthatarerecruitedbythebindingpartnerRAPTOR.Actsbyactivatingtranscription,proteinsynthesisandribosomebiogenesis,andinhibitingmRNAdegradationandautophagy.
Immunogen		KLH-conjugatedsyntheticpeptidederivedfromArabidopsisthalianaTORproteinsequence,UniProt:Q9FR53,TAIR:AT1G50030
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		AffinitypurifiedseruminPBSpH7.4.
Format		Lyophilized
Quantity		50µg
Reconstitution		Forreconstitutionadd50µlofsterilewater.
Storage		Storelyophilized/reconstitutedat-20°C;oncereconstitutedmakealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes.
Testedapplications		WesternBlot(WB)
Relatedproducts		AS152831 \|anti-ATG4\|Autophagyprotein,rabbitantibodies AS142769 \|anti-ATG8\|Autophagy-relatedprotein,rabbitantibodies AS132664 \| S6K1-2\|Ribosomal-proteinS6kinasehomolog1,2-phosphorylated,rabbitantibody collectionofantibodiestoproteinsinvolvedintranscription collectionofantibodiestoproteinsinvolvedintranslation Plantproteinextractionbuffer Secondaryantibodies
Additionalinformation

applicationinformation
Recommendeddilution		1:5000(WB)
Expected\|apparentMW		279kDa
Confirmedreactivity		Arabidopsisthaliana,Zeamays
Predictedreactivity		Glycinemax,Oryzasativa,Physcomitrellapatens,Populustrichocarpa,Setariaitalica,Sorghumbicolor,Vitisvinifera
Notreactivein		Chlamydomonasreinhardtii
Additionalinformation		TORisasubjectedtopost-translationalmodifications(ubiquitinationandphosphorylation),whichcanchangethemigrationpatterninSDSgel.
Selectedreferences		Garciaetal.(2017).MaizedefectivekernelmutantgeneratedbyinsertionofaDselementinageneencodingahighlyconservedTTI2cochaperone.ProcNatlAcadSciUSA.2017May16;114(20):5165-5170.doi:10.1073/pnas.1703498114.

applicationinformation

10ofArabidopsisthaliana10dag(day-after-germination)seedlingsgrownonMSagarplatewerehomogenisedin1.5Eppis(safe-lock)inPrecellysmachine(glassbeads+N2liquid)2x7""atmaxspeed.100uLof1.5x(Laemmlibuffer+10%BME)wasaddedandsampleswereboiledat95Cfor5minutesfollowedbya15minutescentrifugationatmaxrpm,wasloadedintheamountof40uLperlaneandseparatedon8%SDS-PAGE.Blottingwasdonefor1hat100V toPVDFusingtanktransfer.Blotswereblockedwith 2%BSAin1xPBS-Tween200.1%for1hatRTwithagitation.Blotwasincubatedintheprimaryantibodyatadilutionof1:5000in1xPBS-Tween200.1%overnightat4Cwithagitation.Theantibodysolutionwasdecantedandtheblotwasbrieflyrinsed,thenwashed3timesfor10minin1xPBS-Tween200.1%atRTwithagitation.Blotwasincubatedinsecondaryantibody(anti-rabbitIgGhorserADIshperoxidaseconjugated)dilutedto1:20000in1xPBS-Tween200.1%for2hatRTwithagitation.Theblotwaswashedasaboveanddevelopedfor1minutewithLumi-Light^PLUSWesternBlottingSubstrate(Roche).Exposuretimewas 30seconds.

CourtesyofDr.MikhailSchepetilnikov,InstitutdeBIOLOGieMoléculairedesPlantes,CNRS,France

immunoprecipitation using anti-plant TOR antibodies

immunoprecipitation using anti-plant TOR antibodies

25mgtotalproteinextract(35S:Raptor-ProtG)wasincubatedwithIgGmagneticbeadsfor50minutes.Afterwashing,complexeswereeluted,concentratedbyspeedvacandpelletwasdissolvedin30uL1xsamplebuffer.ForSDS-PAGE10uLoftheeluatewasloadedongel.20,40,and60μgtotalproteinextractfromArabidopsisthalianacellsUSPensionculture(Landsbergerecta),orAtTORproteinenrichedbyimmunoprecipitationusingAtRAPTOR1Bwasseparatedon4-15%gradientTGX(Biorad)SDS-PAGEandblotted15min.toPVDFusingtheTrans-BlotTurbosystem(settinghighMW).Asnegativecontrol,anunrelatedimmunoprecipitatedsamplewasloaded.Blotswereblockedovernightwith1%PVP40inTBS-Tat4°Cwithagitation.Blotwasincubatedwiththeprimaryanti-TORantibodyatadilutionof1:1000inblockingbufferfor4hatroomtemperature(RT)withagitation.Theantibodysolutionwasdecantedandtheblotwasrinsedbrieflyonce,thenwashedoncefor15minand3timesfor5mininTBS-TatRTwithagitation.Blotwasincubatedinsecondaryantibody(anti-rabbitIgGhorseradishperoxidaseconjugated,fromAgrisera AS09602)dilutedto1:20000inblockingbufferfor1hatRTwithagitation.Theblotwaswashedasaboveanddevelopedfor1minwithECLaccordingtothemanufacturer"sinstructions.Exposuretimewas10seconds.

TORprotein(redarrow)detectedafterenrichmentbyimmunoprecipitationwithIgGbeadsandRaptor-ProtGfusion,whileTORbandisabsentincontrolIPwithunrelatedProtG-taggedbait.

品牌介绍

瑞典Agrisera公司成立于1980年，长期以来，公司致力于植物/环境科学研究中所需蛋白抗体研发与销售，主要集中于模式之物及其他植物的生理、病理相关的蛋白抗体。Agrisera 公司以提供高品质、优服务在欧洲树立了优秀的品牌形象，并成为瑞典 Umeå Plant Science Centre的抗体供货商，同时还积极拓展全球市场，每年都有大批采用该公司抗体产品的高质量研究论文发表于各类学术刊物，受到全球科研工作者的一致认可。Agrisera的所有抗体分两大类：植物及藻类的抗体，以及细菌、真菌、昆虫、鱼等动物类抗体，植物领域的抗体涵盖Arabidopsis thaliana、Hordeum vulgare、Chlamydomonas reinhardtii、Cyanobacteria、Physcomitrella patens、Oryza sativa、Populus sp、Conifers等多个物种。

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Antibodies for Human/Animal

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