product information
Background

Hsp16.6 belongs to a family of class I of a small heat shock proteins. They are induced once a cell is stressed by an increased temperature. Synechocystis Hsp16.6 is involved in the development of thermotolerance and thylakoid stability. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation.
Immunogen

Recombinant protein. Synechocystis PCC 6803 Hsp16.6 CI (class one) P72977
Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications western blot (WB)
Related products

AS08 350 | anti-DnaK2 | heat shock protein 70-2 (HSP70-2)

collection of antibodies to heat shock proteins

Additional information

This product can be sold containing proclin if requested
application information
Recommended dilution

1: 5000 with standard ECL (WB)
Expected | apparent MW

16.6 kDa
Confirmed reactivity Synechocystis sp. PCC 6803
Predicted reactivity

Synechococcus sp., Thermosynechococcus sp.
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment
Additional information

on a gradient gel Hsp16.6 runs closer to 11 kDa
Selected references Gunnelius et al. (2014). The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria. Nucleic Acids Res. 2014 Jan 29.

application example

Left panel: purified recombinant Hsp16.6 in 0.5, 1 and 2 ng per well. Right panel: 5 µg of total protein from Synechocystis PCC 6803 null mutant and WT were separated on 9-17 % SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 5 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody (1: 10 000 from Abcam).

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